recombinant murine tweak  (R&D Systems)

Journal: Redox Biology

Article Title: Macrophage AMPK activated by oxidative stress drives profibrotic crosstalk with tubular cells to accelerate renal fibrosis after ischemic and reperfusion injury

doi: 10.1016/j.redox.2025.104002

Figure Lengend Snippet: Arg1 + MMP12 + macrophage-derived TWEAK licenses PDGFB production in fibroTECs via Fn14 signaling. (A) Ligand-receptor communication analysis between different cell types in IRI kidneys. (B) The major incoming signaling of different cell types. (C) Bubble plots showing the expression of specific ligand-receptor pairs in the interaction between myeloid cell subsets and TEC subsets. (D) Bubble plots showing the expression of specific ligand-receptor pairs in the interaction between TEC subsets and myeloid cell subsets. (E, F) WT/AMPKα1 −/− BMDMs were treated with IL-4 and IL-13, and the expression levels of Arg 1 , Mmp12 , and Tnfsf12 were analyzed by qPCR (n = 5). (G, H) TCMK-1 cells were treated with CM from IL-4 and IL-13-stimulated WT/AMPKα1 −/− BMDMs. The expression of Pdgfb , Cxcl1 , and Egfr in TCMK-1 cells was analyzed by qPCR (n = 5). (I, J) TCMK-1 cells were treated with CM from IL-4 and IL-13-stimulated WT/AMPKα1 −/− BMDMs or together with recombinant TWEAK (rTWEAK). The expression of Pdgfb in TCMK-1 cells was analyzed by qPCR (n = 5). (K, L) TCMK-1 cells were treated with rTWEAK or rTWEAK and an Fn14 inhibitor. The expression of Pdgfb in TCMK-1 cells was detected by qPCR (n = 5). (M) Immunoblots and quantification of IKK-β and nuclear NF-κB p65 in TCMK-1 cells treated with PBS or rTWEAK (n = 3). The results represent mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: To examine the role of the TWEAK-Fn14 axis, cells were treated with recombinant TWEAK (MCE, HY-P7309, 100 ng/ml) and the Fn14 inhibitor, L524-0366 (Sigma, 509374, 10 μM).

Techniques: Derivative Assay, Expressing, Recombinant, Western Blot

Journal: Respiratory Research

Article Title: Plasma proteomic and machine learning models for differentiating idiopathic pulmonary fibrosis and connective tissue disease–associated interstitial lung disease: findings from a prospective cohort

doi: 10.1186/s12931-026-03596-4

Figure Lengend Snippet: Independent protein-level validation of candidate biomarkers in CTD-ILD and IPF. A Plasma levels of TWEAK, ( B ) MMP-10, and ( C ) ADA were quantified by ELISA in an independent validation cohort (CTD-ILD, n = 38; IPF, n = 22). Statistical significance was assessed using the Mann–Whitney U test (*** p < 0.001)

Article Snippet: FGF-19 concentrations were determined using the Human FGF-19 ELISA kit (DY969, R&D Systems), and TWEAK levels were quantified using the Human TWEAK ELISA kit (DY1090, R&D Systems).

Techniques: Biomarker Discovery, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: Molecular Neurobiology

Article Title: In Vitro Analysis of TWEAK/Fn14 Axis in the Blood–Brain Barrier Models during Oxygen–Glucose Deprivation and Reoxygenation

doi: 10.1007/s12035-026-05691-5

Figure Lengend Snippet: Endothelial cells were subjected to different OGD durations with or without 24h reoxygenation (RO) ( n = 3). A Representative Western blot showing TWEAK, Fn14, VE-cadherin, ZO-1, and β-actin expressions. The quantification of protein levels was normalized to β-actin, and relative mRNA expression was normalized to CANX. B Fn14 protein levels, C FN14 mRNA expression, D TWEAK protein levels, E TWEAK mRNA expression, F VE-cadherin protein levels, G ZO-1 protein level, H occludin protein levels. I CDH5 (VE-cadherin) mRNA expression, J TJP1 (ZO-1) mRNA expression, and K relative OCLN (occludin) mRNA expression; columns represent mean values, and the vertical lines denote the standard error of the mean (SEM). * p < 0.05, ** p < 0.01, and *** p < 0.001 between the columns are indicated by horizontal lines

Article Snippet: RT-qPCR was performed using TaqMan probes for the following target genes: (i) OCLN (Hs00170162_m1), (ii) CLDN5 (Hs00533949_s1), (iii) CDH5 (Hs00901465_m1), (iv) TJP1 (Hs01551871_m1), (v) TNFRSF12A (Hs00171993_m1), and (vi) TNFSF12 (Hs00387540_g1).

Techniques: Western Blot, Expressing

Journal: Molecular Neurobiology

Article Title: In Vitro Analysis of TWEAK/Fn14 Axis in the Blood–Brain Barrier Models during Oxygen–Glucose Deprivation and Reoxygenation

doi: 10.1007/s12035-026-05691-5

Figure Lengend Snippet: Astrocyte cells were subjected to different OGD durations, with or without 24h reoxygenation (RO) ( n = 3). A Representative Western blot showing TWEAK, Fn14, VE-cadherin, ZO-1, and β-actin expressions. The quantification of protein levels was normalized to β-actin, and relative mRNA expression was normalized to CANX. B Fn14 protein levels, C FN14 mRNA expression, D TWEAK protein levels, and E TWEAK mRNA expression; columns represent mean values, and vertical lines denote the standard error of the mean (SEM). * p < 0.05 and ** p < 0.01 between the columns are indicated by horizontal lines

Article Snippet: RT-qPCR was performed using TaqMan probes for the following target genes: (i) OCLN (Hs00170162_m1), (ii) CLDN5 (Hs00533949_s1), (iii) CDH5 (Hs00901465_m1), (iv) TJP1 (Hs01551871_m1), (v) TNFRSF12A (Hs00171993_m1), and (vi) TNFSF12 (Hs00387540_g1).

Techniques: Western Blot, Expressing

Journal: Molecular Neurobiology

Article Title: In Vitro Analysis of TWEAK/Fn14 Axis in the Blood–Brain Barrier Models during Oxygen–Glucose Deprivation and Reoxygenation

doi: 10.1007/s12035-026-05691-5

Figure Lengend Snippet: Pericyte cells were subjected to different OGD durations, with or without 24h reoxygenation (RO) ( n = 3). A Representative Western blot showing TWEAK, Fn14, VE-Cadherin, ZO-1, and β-actin expressions. The quantification of protein levels was normalized to β-actin, and relative mRNA expression was normalized to CANX. B Fn14 protein levels, C FN14 mRNA expression, D TWEAK protein levels, and E TWEAK mRNA expression; columns represent mean values, and vertical lines denote the standard error of the mean (SEM). * p < 0.05 and ** p < 0.01 between the columns are indicated by horizontal lines

Article Snippet: RT-qPCR was performed using TaqMan probes for the following target genes: (i) OCLN (Hs00170162_m1), (ii) CLDN5 (Hs00533949_s1), (iii) CDH5 (Hs00901465_m1), (iv) TJP1 (Hs01551871_m1), (v) TNFRSF12A (Hs00171993_m1), and (vi) TNFSF12 (Hs00387540_g1).

Techniques: Western Blot, Expressing

Journal: Molecular Neurobiology

Article Title: In Vitro Analysis of TWEAK/Fn14 Axis in the Blood–Brain Barrier Models during Oxygen–Glucose Deprivation and Reoxygenation

doi: 10.1007/s12035-026-05691-5

Figure Lengend Snippet: Endothelial cells and pericytes were subjected to different OGD durations, with or without 24h reoxygenation (RO) ( n = 3). A Representative Western blot showing TWEAK, Fn14, VE-cadherin, ZO-1, and β-actin expressions in endothelial cells and pericytes. The quantification of protein levels was normalized to β-actin, and relative mRNA expression was normalized to CANX. The quantification of Fn14 and TWEAK protein levels in endothelial cells ( B–C ) and pericytes ( D–E ); relative FN14 and TWEAK mRNA expression in endothelial cells ( F–G ) and pericytes ( H–I ); the quantification of additional protein levels of VE-cadherin ( J ), ZO-1 ( K ), and occludin ( L ); and relative mRNA expression of CDH5 (VE-cadherin) ( M ) TJP1 (ZO-1) ( N ), and OCLN (occludin) ( O ). Columns represent mean values, and vertical lines denote the standard error of the mean (SEM). * p < 0.05, ** p < 0.01, and *** p < 0.001 between the columns are indicated by horizontal lines

Article Snippet: RT-qPCR was performed using TaqMan probes for the following target genes: (i) OCLN (Hs00170162_m1), (ii) CLDN5 (Hs00533949_s1), (iii) CDH5 (Hs00901465_m1), (iv) TJP1 (Hs01551871_m1), (v) TNFRSF12A (Hs00171993_m1), and (vi) TNFSF12 (Hs00387540_g1).

Techniques: Western Blot, Expressing

Journal: Molecular Neurobiology

Article Title: In Vitro Analysis of TWEAK/Fn14 Axis in the Blood–Brain Barrier Models during Oxygen–Glucose Deprivation and Reoxygenation

doi: 10.1007/s12035-026-05691-5

Figure Lengend Snippet: Endothelial cells and astrocytes were subjected to different OGD durations, with or without 24h reoxygenation (RO) ( n = 3). A Representative Western blot showing TWEAK, Fn14, VE-cadherin, ZO-1, and β-actin expressions in endothelial cells and astrocytes. The quantification of protein levels was normalized to β-actin, and relative mRNA expression was normalized to CANX. The quantification of Fn14 and TWEAK protein levels in endothelial cells ( B–C ) and astrocytes ( D–E ); relative FN14 and TWEAK mRNA expressions in endothelial cells ( F–G ) and astrocytes ( H–I ); the quantification of protein levels of VE-cadherin ( J ), ZO-1 ( K ), and occludin ( L ); and relative mRNA expression of CDH5 (VE-cadherin) ( M ), TJP1 (ZO-1) ( N ), and OCLN (occludin) ( O ). Columns represent mean values, and vertical lines denote the standard error of the mean (SEM). * p < 0.05 and ** p < 0.01 between the columns are indicated by horizontal lines

Article Snippet: RT-qPCR was performed using TaqMan probes for the following target genes: (i) OCLN (Hs00170162_m1), (ii) CLDN5 (Hs00533949_s1), (iii) CDH5 (Hs00901465_m1), (iv) TJP1 (Hs01551871_m1), (v) TNFRSF12A (Hs00171993_m1), and (vi) TNFSF12 (Hs00387540_g1).

Techniques: Western Blot, Expressing

Journal: Molecular Neurobiology

Article Title: In Vitro Analysis of TWEAK/Fn14 Axis in the Blood–Brain Barrier Models during Oxygen–Glucose Deprivation and Reoxygenation

doi: 10.1007/s12035-026-05691-5

Figure Lengend Snippet: Endothelial cells–astrocytes–pericytes were subjected to 4 h of OGD with or without 24h reoxygenation (RO) ( n = 3). A Representative Western blot showing TWEAK, Fn14, VE-Cadherin, ZO-1, and β-actin expressions in endothelial cells and astrocytes–pericytes. The quantification of protein levels was normalized to β-actin, and relative mRNA expression was normalized to CANX. The quantification of Fn14 and TWEAK protein levels in endothelial cells ( B–C ) and astrocytes–pericytes ( D – E ); relative FN14 and TWEAK mRNA expression in endothelial cells ( F–G ) and astrocytes–pericytes ( H – I ); the quantification of protein levels of VE-cadherin ( J ), ZO-1 ( K ), and occludin ( L ); and relative mRNA expression of CDH5 (VE-cadherin) ( M ), TJP1 (ZO-1) ( N ), and OCLN (occludin) ( O ). Columns represent mean values and vertical lines denote the standard error of the mean (SEM). * p < 0.05 and ** p < 0.01 between the columns are indicated by horizontal lines

Article Snippet: RT-qPCR was performed using TaqMan probes for the following target genes: (i) OCLN (Hs00170162_m1), (ii) CLDN5 (Hs00533949_s1), (iii) CDH5 (Hs00901465_m1), (iv) TJP1 (Hs01551871_m1), (v) TNFRSF12A (Hs00171993_m1), and (vi) TNFSF12 (Hs00387540_g1).

Techniques: Western Blot, Expressing